PUBLICATION

Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants

Authors
Traver, D., Paw, B.H., Poss, K.D., Penberthy, W.T., Lin, S., and Zon, L.I.
ID
ZDB-PUB-031111-3
Date
2003
Source
Nature immunology   4(12): 1238-1246 (Journal)
Registered Authors
Lin, Shuo, Pawar, Dilip P., Penberthy, W. Todd, Poss, Kenneth D., Traver, David, Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals, Genetically Modified
  • Hematopoietic Stem Cell Transplantation
  • Cell Lineage*
  • Animals
  • Zebrafish
  • Flow Cytometry
  • Hematopoietic Stem Cells/physiology*
  • Kidney/cytology
  • Kidney/physiology
  • Mutation
  • Spleen/cytology
  • Spleen/physiology
  • Blood Physiological Phenomena
  • Blood Cells/cytology
  • Blood Cells/physiology
  • Hematopoietic System/embryology*
  • Hematopoietic System/growth & development*
(all 17)
PubMed
14608381 Full text @ Nat. Immunol.
Abstract
The zebrafish is firmly established as a genetic model for the study of vertebrate blood development. Here we have characterized the blood-forming system of adult zebrafish. Each major blood lineage can be isolated by flow cytometry, and with these lineal profiles, defects in zebrafish blood mutants can be quantified. We developed hematopoietic cell transplantation to study cell autonomy of mutant gene function and to establish a hematopoietic stem cell assay. Hematopoietic cell transplantation can rescue multilineage hematopoiesis in embryonic lethal gata1(-/-) mutants for over 6 months. Direct visualization of fluorescent donor cells in embryonic recipients allows engraftment and homing events to be imaged in real time. These results provide a cellular context in which to study the genetics of hematopoiesis.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la2TgTransgenic Insertion
    la3TgTransgenic Insertion
      la781TgTransgenic Insertion
        sd2TgTransgenic Insertion
          zdf8TgTransgenic Insertion
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            Fish
            la2Tg
            la3Tg
            la781Tg
            sd2Tg
            zdf8Tg
            1 - 5 of 5
            Show
            Antibodies
            Orthology
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            1 - 1 of 1
            Show
            Mapping
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            Citation
            Traver, D., Paw, B.H., Poss, K.D., Penberthy, W.T., Lin, S., and Zon, L.I. (2003) Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nature immunology. 4(12):1238-1246.