PUBLICATION
A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish
- Authors
- Bae, Y.K., Shimizu, T., Yabe, T., Kim, C.H., Hirata, T., Nojima, H., Muraoka, O., Hirano, T., and Hibi, M.
- ID
- ZDB-PUB-030319-2
- Date
- 2003
- Source
- Development (Cambridge, England) 130(9): 1853-1865 (Journal)
- Registered Authors
- Bae, Young Ki, Hibi, Masahiko, Hirata, Tsutomu, Kim, Cheol-Hee, Muraoka, Osamu, Nojima, Hideaki, Shimizu, Takashi, Yabe, Taijirou
- Keywords
- none
- MeSH Terms
-
- Receptors, Notch
- Transcription, Genetic/physiology
- Gene Expression Regulation, Developmental/physiology
- Amino Acid Sequence
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Molecular Sequence Data
- Transcription Factors*
- Genes, Homeobox*
- Nuclear Proteins*
- Phylogeny
- Nerve Tissue Proteins/genetics
- Signal Transduction/physiology
- Membrane Proteins/metabolism
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism
- Nervous System/embryology*
- Animals
- Zebrafish/embryology*
- PubMed
- 12642490 Full text @ Development
Citation
Bae, Y.K., Shimizu, T., Yabe, T., Kim, C.H., Hirata, T., Nojima, H., Muraoka, O., Hirano, T., and Hibi, M. (2003) A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish. Development (Cambridge, England). 130(9):1853-1865.
Abstract
A homeobox gene, pnx, is expressed in prospective posterior neurogenic regions and later in primary neurons. pnx expression was regulated by a signal from the non-axial mesendoderm and by Notch signaling. Pnx contains an Eh1 repressor domain, which interacted with Groucho and acted as a transcriptional repressor. Misexpression of pnx increased neural precursor cells and postmitotic neurons, which express neurogenin1 and elavl3/HuC, respectively. Expression of an antimorphic Pnx (VP16Pnx) or inhibition of Pnx by antisense morpholino oligonucleotide led to the reduction in the number of a subset of primary neurons. Misexpression of pnx promoted neurogenesis independent of Notch signaling. Epistatic analyses showed that Pnx also functions downstream of the Notch signal. These data indicate that pnx is a novel repressor-type homeobox gene that regulates posterior neurogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping