PUBLICATION

Eliminating zebrafish Pbx proteins reveals a hindbrain ground state

Authors
Waskiewicz, A.J., Rikhof, H.A., and Moens, C.B.
ID
ZDB-PUB-021107-1
Date
2002
Source
Developmental Cell   3(5): 723-733 (Journal)
Registered Authors
Moens, Cecilia, Rikhof, Holly, Waskiewicz, Andrew
Keywords
none
MeSH Terms
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology*
  • Rhombencephalon/embryology*
  • DNA, Complementary
  • Animals
  • DNA-Binding Proteins*
  • Molecular Sequence Data
  • Base Sequence
  • Mosaicism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Neurons
(all 14)
PubMed
12431378 Full text @ Dev. Cell
Abstract
The vertebrate hindbrain is divided into serially homologous segments, the rhombomeres (r). Pbx and Hox proteins are hypothesized to form heterodimeric, DNA binding transcription complexes which specify rhombomere identities. Here, we show that eliminating zebrafish Lzr/Pbx4 and Pbx2 function prevents hindbrain segmentation and causes a wholesale anterior homeotic transformation of r2-r6, to r1 identity. We demonstrate that Pbx proteins interact with Hox paralog group 1 proteins to specify segment identities broadly within the hindbrain, and that this process involves the Pbx:Hox-1-dependent induction of Fgf signals in r4. We propose that in the absence of Pbx function, r2-r6 acquire a homogeneous ground state identity, that of r1, and that Pbx proteins, functioning primarily with their Hox partners, function to modify this ground state identity during normal hindbrain development.
Genes / Markers
Figures
No images available
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
egr2bpbx4b557/b557standard conditions1-4 somitesISH
egr2bpbx4b557/b557standard conditions14-19 somitesISH
egr2bpbx4b557/b557standard conditions20-25 somitesISH
egr2bWTstandard conditions20-25 somitesISH
egr2bWTstandard conditions1-4 somitesISH
egr2bWTstandard conditions14-19 somitesISH
epha4apbx4b557/b557standard conditions20-25 somitesISH
epha4aWTstandard conditions20-25 somitesISH
fgfr3pbx4b557/b557standard conditions20-25 somitesISH
fgfr3WTstandard conditions20-25 somitesISH
1 - 10 of 30
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b557
    		Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    hoxb1bMO2-hoxb1bMRPHLNO
    pbx2MO1-pbx2MRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    pbx4b557/b557
    WT
    1 - 2 of 2
    Show
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Waskiewicz, A.J., Rikhof, H.A., and Moens, C.B. (2002) Eliminating zebrafish Pbx proteins reveals a hindbrain ground state. Developmental Cell. 3(5):723-733.