PUBLICATION

Eliminating zebrafish Pbx proteins reveals a hindbrain ground state

Authors
Waskiewicz, A.J., Rikhof, H.A., and Moens, C.B.
ID
ZDB-PUB-021107-1
Date
2002
Source
Developmental Cell   3(5): 723-733 (Journal)
Registered Authors
Moens, Cecilia, Rikhof, Holly, Waskiewicz, Andrew
Keywords
none
MeSH Terms
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology*
  • Rhombencephalon/embryology*
  • DNA, Complementary
  • Animals
  • DNA-Binding Proteins*
  • Molecular Sequence Data
  • Base Sequence
  • Mosaicism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Neurons
(all 14)
PubMed
12431378 Full text @ Dev. Cell
Abstract
The vertebrate hindbrain is divided into serially homologous segments, the rhombomeres (r). Pbx and Hox proteins are hypothesized to form heterodimeric, DNA binding transcription complexes which specify rhombomere identities. Here, we show that eliminating zebrafish Lzr/Pbx4 and Pbx2 function prevents hindbrain segmentation and causes a wholesale anterior homeotic transformation of r2-r6, to r1 identity. We demonstrate that Pbx proteins interact with Hox paralog group 1 proteins to specify segment identities broadly within the hindbrain, and that this process involves the Pbx:Hox-1-dependent induction of Fgf signals in r4. We propose that in the absence of Pbx function, r2-r6 acquire a homogeneous ground state identity, that of r1, and that Pbx proteins, functioning primarily with their Hox partners, function to modify this ground state identity during normal hindbrain development.
Genes / Markers
Figures
No images available
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b557
    Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    hoxb1bMO2-hoxb1bMRPHLNO
    pbx2MO1-pbx2MRPHLNO
    1 - 2 of 2
    Show
    Fish
    1 - 2 of 2
    Show
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available