PUBLICATION

Zebrafish mutants identify an essential role for laminins in notochord formation

Authors
Parsons, M.J., Pollard, S.M., Saude, L., Feldman, B., Coutinho, P., Hirst, E.M., and Stemple, D.L.
ID
ZDB-PUB-020716-26
Date
2002
Source
Development (Cambridge, England)   129(13): 3137-3146 (Journal)
Registered Authors
Coutinho, Pedro, Feldman, Benjamin, Parsons, Michael, Saude, Leonor, Stemple, Derek L.
Keywords
laminin; notochord; basement membrane; differentiation; zebrafish
MeSH Terms
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Notochord/embryology*
  • Molecular Sequence Data
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Embryo, Nonmammalian
  • Animals
  • Cell Differentiation/genetics
  • Mesoderm
  • Laminin/genetics*
  • Laminin/metabolism
  • Gene Expression Regulation, Developmental
  • Mutation
(all 14)
PubMed
12070089 Full text @ Development
Abstract
Basement membranes are thought to be essential for organ formation, providing the scaffold on which individual cells organize to form complex tissues. Laminins are integral components of basement membranes. To understand the development of a simple vertebrate organ, we have used positional cloning to characterize grumpy and sleepy, two zebrafish loci known to control notochord formation, and find that they encode laminin beta1 and laminin gamma1, respectively. Removal of either chain results in the dramatic loss of laminin 1 staining throughout the embryo and prevents formation of the basement membrane surrounding the notochord. Notochord cells fail to differentiate and many die by apoptosis. By transplantation, we demonstrate that, for both grumpy and sleepy, notochord differentiation can be rescued by exogenous sources of the missing laminin chain, although notochordal sources are also sufficient for rescue. These results demonstrate a clear in vivo requirement for laminin beta1 and laminin gamma1 in the formation of a specific vertebrate organ and show that laminin or the laminin-dependent basement membrane is essential for the differentiation of chordamesoderm to notochord.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m86
    Point Mutation
    m189
      Point Mutation
      m466
        Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        lamb1aMO1-lamb1aMRPHLNO
        lamb4MO1-lamb4MRPHLNO
        lamc1MO2-lamc1MRPHLNO
        lamc1MO3-lamc1MRPHLNO
        1 - 4 of 4
        Show
        Fish
        Antibodies
        Name Type Antigen Genes Isotypes Host Organism
        Ab1-lama1polyclonal
          Rabbit
          1 - 1 of 1
          Show
          Orthology
          Gene Orthology
          lamb1a
          lamb4
          lamc1
          1 - 3 of 3
          Show
          Engineered Foreign Genes
          No data available
          Mapping
          Entity Type Entity Symbol Location
          GENEelavl1aChr: 2 Details
          GENElamb1aChr: 25 Details
          GENElamb4Chr: 25 Details
          GENElamc1Chr: 2 Details
          GENEmyod1Chr: 25 Details
          SSLPz7632Chr: 2 Details
          SSLPz11023Chr: 2 Details
          1 - 7 of 7
          Show