PUBLICATION

Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning

Authors
Lekven, A.C., Thorpe, C.J., Waxman, J.S., and Moon, R.T.
ID
ZDB-PUB-011116-4
Date
2001
Source
Developmental Cell   1(1): 103-114 (Journal)
Registered Authors
Lekven, Arne, Moon, Randall T., Thorpe, Chris, Waxman, Joshua
Keywords
none
MeSH Terms
  • Body Patterning/physiology*
  • Animals
  • Cytoskeletal Proteins
  • Transcription, Genetic/physiology*
  • Zebrafish/embryology*
  • Fetal Proteins
  • Head/embryology
  • T-Box Domain Proteins/genetics
  • Amino Acid Sequence
  • Molecular Sequence Data
  • Tail/embryology
  • Mutagenesis/physiology
  • Mesoderm/physiology
  • Zebrafish Proteins/genetics*
  • Open Reading Frames
  • Proteins/genetics*
  • Ectoderm/physiology
  • Oligonucleotides, Antisense
  • Gene Expression Regulation, Developmental
  • RNA, Messenger/genetics
  • Phenotype
  • Wnt Proteins
(all 22)
PubMed
11703928 Full text @ Dev. Cell
Abstract
In vertebrates, wnt8 has been implicated in the early patterning of the mesoderm. To determine directly the embryonic requirements for wnt8, we generated a chromosomal deficiency in zebrafish that removes the bicistronic wnt8 locus. We report that homozygous mutants exhibit pronounced defects in dorso-ventral mesoderm patterning and in the antero-posterior neural pattern. Despite differences in their signaling activities, either coding region of the bicistronic RNA can rescue the deficiency phenotype. Specific interference of wnt8 translation by morpholino antisense oligomers phenocopies the deficiency, and interference with wnt8 translation in ntl and spt mutants produces embryos lacking trunk and tail. These data demonstrate that the zebrafish wnt8 locus is required during gastrulation to pattern both the mesoderm and the neural ectoderm properly.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+standard conditionsPrim-5
Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+standard conditionsPrim-5
Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+standard conditionsPrim-5
Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+standard conditionsPrim-5
Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+standard conditionsPrim-5
Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+standard conditionsPrim-5
Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+standard conditionsPrim-5
Df(Chr14:wnt8a)w8/w8standard conditions30%-epiboly to Bud
Df(Chr14:wnt8a)w8/w8standard conditions30%-epiboly to Bud
Df(Chr14:wnt8a)w8/w8standard conditions30%-epiboly to Bud
1 - 10 of 39
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    b160
      		Indel
      b195
        		Insertion
        w8
          		Deficiency
          w11
            		Translocation
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            wnt8aMO1-wnt8aMRPHLNO
            wnt8aMO2-wnt8aMRPHLNO
            1 - 2 of 2
            Show
            Fish
            Fish
            Df(Chr14:wnt8a)w8/+; T(Chr14:wnt8a)w11/+
            Df(Chr14:wnt8a)w8/w8
            tbx16b104/b104 (AB)
            tbx16b104/b104 + MO1-wnt8a + MO2-wnt8a (AB)
            tbxtab160/b160 (AB)
            tbxtab160/b160 + MO1-wnt8a + MO2-wnt8a (AB)
            tbxtab195/b195
            T(Chr14:wnt8a)w11/w11
            WT + MO1-wnt8a
            WT + MO1-wnt8a + MO2-wnt8a
            1 - 10 of 11
            Show
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            No data available
            Mapping
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            Citation
            Lekven, A.C., Thorpe, C.J., Waxman, J.S., and Moon, R.T. (2001) Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning. Developmental Cell. 1(1):103-114.