PUBLICATION

Zebrafish smoothened functions in ventral neural tube specification and axon tract formation

Authors
Varga, Z.M., Amores, A., Lewis, K.E., Yan, Y.-L., Postlethwait, J.H., Eisen, J.S., and Westerfield, M.
ID
ZDB-PUB-010927-3
Date
2001
Source
Development (Cambridge, England)   128(18): 3497-3509 (Journal)
Registered Authors
Amores, Angel, Eisen, Judith S., Lewis, Katharine E., Postlethwait, John H., Varga, Zoltán M., Westerfield, Monte, Yan, Yi-Lin
Keywords
hedgehog; forebrain patterning; spinal cord; floor plate; motoneurons; pituitary ventral neural tube; zebrafish
MeSH Terms
  • Motor Neurons
  • Molecular Sequence Data
  • Mutation
  • Pituitary Gland, Anterior/cytology
  • Pituitary Gland, Anterior/embryology
  • Trans-Activators/metabolism
  • Body Patterning*
  • Receptors, G-Protein-Coupled*
  • Retina/cytology
  • Retina/embryology
  • Phenotype
  • Zebrafish Proteins/genetics
  • Spinal Cord/cytology
  • Spinal Cord/embryology
  • Signal Transduction
  • Visual Pathways/cytology
  • Visual Pathways/embryology
  • Zebrafish/embryology*
  • Nervous System/cytology
  • Nervous System/embryology*
  • Transcription Factors/genetics
  • Prosencephalon/cytology
  • Prosencephalon/embryology
  • Hedgehog Proteins
  • Animals
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism*
(all 27)
PubMed
11566855 Full text @ Development
Abstract
Sonic hedgehog (Shh) signaling patterns many vertebrate tissues. shh mutations dramatically affect mouse ventral forebrain and floor plate but produce minor defects in zebrafish. Zebrafish have two mammalian Shh orthologs, sonic hedgehog and tiggy-winkle hedgehog, and another gene, echidna hedgehog, that could have overlapping functions. To examine the role of Hedgehog signaling in zebrafish, we have characterized slow muscle omitted (smu) mutants. We show that smu encodes a zebrafish ortholog of Smoothened that transduces Hedgehog signals. Zebrafish smoothened is expressed maternally and zygotically and supports specification of motoneurons, pituitary cells and ventral forebrain. We propose that smoothened is required for induction of lateral floor plate and a subpopulation of hypothalamic cells and for maintenance of medial floor plate and hypothalamic cells.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b577
    Point Mutation
    b641
      Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      1 - 3 of 3
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      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      zl-1monoclonal
        IgG1Mouse
        zn-5monoclonalIgG1Mouse
        1 - 2 of 2
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        Orthology
        Gene Orthology
        smo
        1 - 1 of 1
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        Engineered Foreign Genes
        No data available
        Mapping
        No data available