PUBLICATION

Zebrafish deadly seven functions in neurogenesis

Authors
Gray, M., Moens, C.B., Amacher, S.L., Eisen, J.S., and Beattie, C.E.
ID
ZDB-PUB-010813-2
Date
2001
Source
Developmental Biology   237(2): 306-323 (Journal)
Registered Authors
Amacher, Sharon, Beattie, Christine, Eisen, Judith S., Gray, Michelle, Moens, Cecilia
Keywords
mutational analysis; primary motoneurons; neural crest; Rohon-Beard neurons; somitogenesis; Mauthner cells; reticulospinal neurons; lateral inhibition; Notch
MeSH Terms
  • Nervous System/embryology*
  • Bromodeoxyuridine/metabolism
  • Neurons/metabolism
  • Neurons/physiology*
  • Receptors, Notch
  • Immunohistochemistry
  • Plasmids/metabolism
  • In Situ Hybridization
  • Receptor, Notch1
  • RNA/metabolism
  • Xenopus
  • Antibodies, Monoclonal/metabolism
  • Zebrafish
  • Alleles
  • Phenotype
  • Mutation
  • Genetic Complementation Test
  • Membrane Proteins/metabolism
  • Neural Crest/metabolism
  • Gene Expression Regulation, Developmental*
  • Time Factors
  • Signal Transduction
  • Homeodomain Proteins/biosynthesis*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology*
  • Zebrafish Proteins
  • Animals
  • Nerve Tissue Proteins/biosynthesis*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology*
  • Somites/metabolism
(all 31)
PubMed
11543616 Full text @ Dev. Biol.
Abstract
In a genetic screen, we isolated a mutation that perturbed motor axon outgrowth, neurogenesis, and somitogenesis. Complementation tests revealed that this mutation is an allele of deadly seven (des). By creating genetic mosaics, we demonstrate that the motor axon defect is non-cell autonomous. In addition, we show that the pattern of migration for some neural crest cell populations is aberrant and crest-derived dorsal root ganglion neurons are misplaced. Furthermore, our analysis reveals that des mutant embryos exhibit a neurogenic phenotype. We find an increase in the number of primary motoneurons and in the number of three hindbrain reticulospinal neurons: Mauthner cells, RoL2 cells, and MiD3cm cells. We also find that the number of Rohon-Beard sensory neurons is decreased whereas neural crest-derived dorsal root ganglion neurons are increased in number supporting a previous hypothesis that Rohon-Beard neurons and neural crest form an equivalence group during development. Mutations in genes involved in Notch-Delta signaling result in defects in somitogenesis and neurogenesis. We found that overexpressing an activated form of Notch decreased the number of Mauthner cells in des mutants indicating that des functions via the Notch-Delta signaling pathway to control the production of specific cell types within the central and peripheral nervous systems.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
notch1ab420/b420 (AB)standard conditions5-9 somites
notch1ab420/b420 (AB)standard conditions10-13 somites
notch1ab420/b420 (AB)standard conditions14-19 somites
notch1ab420/b420 (AB)standard conditions14-19 somites
notch1ab420/b420 (AB)standard conditions20-25 somites
notch1ab420/b420 (AB)standard conditions20-25 somites
notch1ab420/b420 (AB)standard conditions20-25 somites
notch1ab420/b420 (AB)standard conditionsPrim-5
notch1ab420/b420 (AB)standard conditionsPrim-5
notch1ab420/b420 (AB)standard conditionsPrim-5
1 - 10 of 38
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b420
    		Point Mutation
    b638
      		Unknown
      tp37
        		Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        notch1ab420/b420 (AB)
        notch1ab638/b638 (AB)
        notch1atp37/tp37 (AB)
        1 - 3 of 3
        Show
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Gray, M., Moens, C.B., Amacher, S.L., Eisen, J.S., and Beattie, C.E. (2001) Zebrafish deadly seven functions in neurogenesis. Developmental Biology. 237(2):306-323.