PUBLICATION

A crucial component of the endoderm formation pathway, CASANOVA, is encoded by a novel sox-related gene

Authors
Dickmeis, T., Mourrain, P., Saint-Etienne, L., Fischer, N., Aanstad, P., Clark, M., Strähle, U., and Rosa, F.
ID
ZDB-PUB-010706-4
Date
2001
Source
Genes & Development   15(12): 1487-1492 (Journal)
Registered Authors
Aanstad, Pia, Clark, Matthew D., Dickmeis, Thomas, Fischer, Nadine, Mourrain, Philippe, Rosa, Frederic, Saint-Etienne, Laure, Strähle, Uwe
Keywords
casanova; endoderm; nodal; cloning; zebrafish; sox
MeSH Terms
  • Proteins/genetics
  • Zebrafish Proteins*
  • Receptors, Transforming Growth Factor beta/genetics
  • Receptors, Transforming Growth Factor beta/metabolism
  • Stem Cells/cytology
  • Zebrafish
  • Nodal Protein
  • Amino Acid Sequence
  • Protein Serine-Threonine Kinases*
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/metabolism*
  • High Mobility Group Proteins/physiology*
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
  • SOX Transcription Factors
  • DNA-Binding Proteins*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Transcription Factors/physiology*
  • Humans
  • Molecular Sequence Data
  • Cell Differentiation
  • Animals
  • Endoderm/cytology
  • Endoderm/physiology*
  • Gastrula/physiology
  • Gene Expression Profiling
  • Signal Transduction*
  • SOXF Transcription Factors
  • Mesoderm/cytology
  • Mesoderm/physiology*
(all 31)
PubMed
11410529 Full text @ Genes & Dev.
Abstract
casanova (cas) mutant zebrafish embryos lack endoderm and develop cardia bifida. In a substractive screen for Nodal-responsive genes, we isolated an HMG box-containing gene, 10J3, which is expressed in the endoderm. The cas phenotype is rescued by overexpression of 10J3 and can be mimicked by 10J3-directed morpholinos. Furthermore, we identified a mutation within 10J3 coding sequence that cosegregates with the cas phenotype, clearly demonstrating that cas is encoded by 10J3. Epistasis experiments are consistent with an instructive role for cas in endoderm formation downstream of Nodal signals and upstream of sox17. In the absence of cas activity, endoderm progenitors differentiate into mesodermal derivatives. Thus, cas is an HMG box-containing gene involved in the fate decision between endoderm and mesoderm that acts downstream of Nodal signals.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m134
    		Point Mutation
    ta56
      		Point Mutation
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Fish
      Fish
      sox32ta56/ta56
      tdgf1m134/m134 (AB)
      1 - 2 of 2
      Show
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
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      Citation
      Dickmeis, T., Mourrain, P., Saint-Etienne, L., Fischer, N., Aanstad, P., Clark, M., Strähle, U., and Rosa, F. (2001) A crucial component of the endoderm formation pathway, CASANOVA, is encoded by a novel sox-related gene. Genes & Development. 15(12):1487-1492.