PUBLICATION

A crucial component of the endoderm formation pathway, CASANOVA, is encoded by a novel sox-related gene

Authors
Dickmeis, T., Mourrain, P., Saint-Etienne, L., Fischer, N., Aanstad, P., Clark, M., Strähle, U., and Rosa, F.
ID
ZDB-PUB-010706-4
Date
2001
Source
Genes & Development   15(12): 1487-1492 (Journal)
Registered Authors
Aanstad, Pia, Clark, Matthew D., Dickmeis, Thomas, Fischer, Nadine, Mourrain, Philippe, Rosa, Frederic, Saint-Etienne, Laure, Strähle, Uwe
Keywords
casanova; endoderm; nodal; cloning; zebrafish; sox
MeSH Terms
  • Animals
  • Signal Transduction*
  • Molecular Sequence Data
  • Gastrula/physiology
  • Zebrafish
  • Humans
  • Mesoderm/cytology
  • Mesoderm/physiology*
  • Gene Expression Profiling
  • SOX Transcription Factors
  • SOXF Transcription Factors
  • Stem Cells/cytology
  • Nodal Protein
  • Zebrafish Proteins*
  • Cell Differentiation
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Transcription Factors/physiology*
  • Endoderm/cytology
  • Endoderm/physiology*
  • Proteins/genetics
  • Protein Serine-Threonine Kinases*
  • DNA-Binding Proteins*
  • Receptors, Transforming Growth Factor beta/genetics
  • Receptors, Transforming Growth Factor beta/metabolism
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/metabolism*
  • High Mobility Group Proteins/physiology*
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
  • Amino Acid Sequence
(all 31)
PubMed
11410529 Full text @ Genes & Dev.
Abstract
casanova (cas) mutant zebrafish embryos lack endoderm and develop cardia bifida. In a substractive screen for Nodal-responsive genes, we isolated an HMG box-containing gene, 10J3, which is expressed in the endoderm. The cas phenotype is rescued by overexpression of 10J3 and can be mimicked by 10J3-directed morpholinos. Furthermore, we identified a mutation within 10J3 coding sequence that cosegregates with the cas phenotype, clearly demonstrating that cas is encoded by 10J3. Epistasis experiments are consistent with an instructive role for cas in endoderm formation downstream of Nodal signals and upstream of sox17. In the absence of cas activity, endoderm progenitors differentiate into mesodermal derivatives. Thus, cas is an HMG box-containing gene involved in the fate decision between endoderm and mesoderm that acts downstream of Nodal signals.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping