PUBLICATION

A regulator of transcriptional elongation controls vertebrate neuronal development

Authors
Guo, S., Yamaguchi, Y., Schilbach, S., Wada, T., Lee, J., Goddard, A., French, D., Handa, H., and Rosenthal, A.
ID
ZDB-PUB-001205-5
Date
2000
Source
Nature   408(6810): 366-369 (Journal)
Registered Authors
Guo, Su
Keywords
none
MeSH Terms
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Repressor Proteins/genetics*
  • Repressor Proteins/physiology
  • Gene Expression Regulation, Developmental*
  • Neurons/physiology*
  • Embryo, Nonmammalian
  • Nuclear Proteins/genetics*
  • Nuclear Proteins/physiology*
  • Transcription, Genetic
  • Zebrafish
  • Amino Acid Substitution
  • Zebrafish Proteins*
  • Animals
  • Mutation
  • Molecular Sequence Data
  • Amino Acid Sequence
  • Transcription Factors/genetics*
  • Transcription Factors/physiology
(all 19)
PubMed
11099044 Full text @ Nature
Abstract
The development of distinct vertebrate neurons is defined by the unique profiles of genes that neurons express. It is accepted that neural genes are regulated at the point of transcription initiation, but the role of messenger RNA elongation in neural gene regulation has not been examined. Here we describe the mutant foggy, identified in a genetic screen for mutations that affect neuronal development in zebrafish, that displayed a reduction of dopamine-containing neurons and a corresponding surplus of serotonin-containing neurons in the hypothalamus. Positional cloning disclosed that Foggy is a brain-enriched nuclear protein that is structurally related to the transcription elongation factor Spt5 (refs 5-12). Foggy is not part of the basic transcription apparatus but a phosphorylation-dependent, dual regulator of transcription elongation. The mutation disrupts its repressive but not its stimulatory activity. Our results provide molecular, genetic and biochemical evidence that negative regulators of transcription elongation control key aspects of neuronal development.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m806
    		Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    supt5hm806/m806
    1 - 1 of 1
    Show
    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping
    Entity Type Entity Symbol Location
    GENEsupt5hChr: 15 Details
    SSLPz470Chr: 15 Details
    SSLPz21452Chr: 15 Details
    1 - 3 of 3
    Show
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    Citation
    Guo, S., Yamaguchi, Y., Schilbach, S., Wada, T., Lee, J., Goddard, A., French, D., Handa, H., and Rosenthal, A. (2000) A regulator of transcriptional elongation controls vertebrate neuronal development. Nature. 408(6810):366-369.