PUBLICATION

Equivalent genetic roles for bmp7/snailhouse and bmp2b/swirl in dorsoventral pattern formation

Authors

Schmid, B., Fürthauer, M., Connors, S.A., Trout, J., Thisse, B., Thisse, C., and Mullins, M.C.

ID

ZDB-PUB-000309-43

Date

2000

Source

Development (Cambridge, England) 127(5): 957-967 (Journal)

Registered Authors

Connors, Stephanie A., Fürthauer, Maximilian, Mullins, Mary C., Schmid, Bettina, Thisse, Bernard, Thisse, Christine, Trout, Jamie

Keywords

dorsoventral; pattern formation; Bmp7; Bmp2b; TGFß; zebrafish

MeSH Terms

Body Patterning/genetics*
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 7
Cloning, Molecular
Zebrafish/embryology*
Zebrafish/genetics
Phenotype
Mutation
Amino Acid Sequence
Gene Deletion
Animals
Chromosome Mapping
Molecular Sequence Data
Zebrafish Proteins*
Embryo, Nonmammalian/physiology*
Transforming Growth Factor beta*
Mutagenesis
Sequence Homology, Amino Acid
Bone Morphogenetic Proteins/chemistry
Bone Morphogenetic Proteins/genetics
Bone Morphogenetic Proteins/physiology*
Recombinant Proteins/chemistry
Transcription, Genetic
Sequence Alignment

(all 24)

PubMed

10662635 Full text @ Development

Abstract

A bone morphogenetic protein (BMP) signaling pathway acts in the establishment of the dorsoventral axis of the vertebrate embryo. Here we demonstrate the genetic requirement for two different Bmp ligand subclass genes for dorsoventral pattern formation of the zebrafish embryo. From the relative efficiencies observed in Bmp ligand rescue experiments, conserved chromosomal synteny, and isolation of the zebrafish bmp7 gene, we determined that the strongly dorsalized snailhouse mutant phenotype is caused by a mutation in the bmp7 gene. We show that the original snailhouse allele is a hypomorphic mutation and we identify a snailhouse/bmp7 null mutant. We demonstrate that the snailhouse/bmp7 null mutant phenotype is identical to the presumptive null mutant phenotype of the strongest dorsalized zebrafish mutant swirl/bmp2b, revealing equivalent genetic roles for these two Bmp ligands. Double mutant snailhouse/bmp7; swirl/bmp2b embryos do not exhibit additional or stronger dorsalized phenotypes, indicating that these Bmp ligands do not function redundantly in early embryonic development. Furthermore, overexpression experiments reveal that Bmp2b and Bmp7 synergize in the ventralization of wild-type embryos through a cell-autonomous mechanism, suggesting that Bmp2b/Bmp7 heterodimers may act in vivo to specify ventral cell fates in the zebrafish embryo.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
bmp2b	bmp2b^ta72a/ta72a	standard conditions	75%-epiboly	ectoderm	ISH	Fig. 5
bmp2b	bmp2b^ta72a/ta72a	standard conditions	75%-epiboly	margin	ISH	Fig. 5
bmp2b	bmp2b^ta72a/ta72a	standard conditions	75%-epiboly	yolk syncytial layer	ISH	Fig. 5
bmp2b	bmp7a^{sb1aub/sb1aub}	standard conditions	75%-epiboly	ectoderm	ISH	Fig. 5
bmp2b	bmp7a^{sb1aub/sb1aub}	standard conditions	75%-epiboly	margin	ISH	Fig. 5
bmp2b	bmp7a^{sb1aub/sb1aub}	standard conditions	75%-epiboly	yolk syncytial layer	ISH	Fig. 5
bmp2b	bmp7a^ty68a/ty68a	standard conditions	75%-epiboly	ectoderm	ISH	Fig. 5
bmp2b	bmp7a^ty68a/ty68a	standard conditions	75%-epiboly	margin	ISH	Fig. 5
bmp2b	bmp7a^ty68a/ty68a	standard conditions	75%-epiboly	yolk syncytial layer	ISH	Fig. 5
bmp2b	smad5^dtc24/dtc24	standard conditions	75%-epiboly	ectoderm	ISH	Fig. 5

1 - 10 of 68

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
bmp7a^{sb1aub/sb1aub}	standard conditions	5-9 somites	whole organism elliptic, abnormal	Fig. 1
bmp7a^{sb1aub/sb1aub}	standard conditions	5-9 somites	whole organism posterior region wholly dorsalized, abnormal	Fig. 1
bmp7a^ty68a/ty68a	standard conditions	5-9 somites	whole organism elliptic, abnormal	Fig. 1
bmp7a^ty68a/ty68a	standard conditions	5-9 somites	whole organism posterior region wholly dorsalized, abnormal	Fig. 1
bmp7a^ty68a/ty68a	standard conditions	Prim-5	whole organism posterior region truncated, abnormal	Fig. 1

1 - 5 of 5

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
dtc24	Point Mutation	smad5
p11	Deficiency	bmp7a
p15	Deficiency	bmp7a
sb1aub	Point Mutation	bmp7a
ta72a	Point Mutation	bmp2b
tc300a	Point Mutation	bmp2b
ty68a	Point Mutation	bmp7a

1 - 7 of 7

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
bmp2b^ta72a/ta72a
bmp2b^tc300a/+; bmp7a^sb1aub/+
bmp2b^{tc300a/tc300a} (TU)
bmp7a^{sb1aub/sb1aub}
bmp7a^{sb1aub/sb1aub}
bmp7a^ty68a/ty68a
Df(Chr11:bmp7a)p11
Df(Chr11:bmp7a)p11/p11
Df(Chr11:bmp7a)p15
Df(Chr11:bmp7a)p15/p15

1 - 10 of 12

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

Schmid et al., 2000 ZDB-PUB-000309-43 PMID:10662635

Equivalent genetic roles for bmp7/snailhouse and bmp2b/swirl in dorsoventral pattern formation

Schmid et al., 2000

ZDB-PUB-000309-43
PMID:10662635