Fig. S3

Fig. S3 CCM^-/- triple knockout shows exacerbated hypomyelination and abnormal myelin. (A) Quantification of the total number of myelinated axons per µm² from the entire cross-section of 8 dpf zebrafish spinal cord in wild type, cntn1b^−/−mag^−/− dKO, cadm4^−/−mag^−/− dKO, and CCM^−/− tKO. (B) SEM images of non-compact and compact myelin sheaths at 8 dpf, identified for quantification in (C). Scale bars are 0.5 µm. (C) Quantification of the percentage of myelinated axons with compact myelin sheaths at 8 dpf. (D) SEM images of abnormal multi-myelin profiles and myelin outfoldings in zebrafish spinal cord cross-section at 8 dpf, identified for quantification in (E and F). Scale bars are 2 µm. (E) Quantification of the percentage of myelinated axons with abnormal multi-myelin profiles at 8 dpf. (F) Quantification of the percentage of myelin sheath outfoldings at 8 dpf. Graphs represent mean values ± SD, analyzed by one-way ANOVA with Tukey’s multiple comparisons test (A), and by Brown-Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test (C, E, F). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data was collected from 5 to 6 fish (A, C, E, F) per genotype. The data underlying this Figure can be found in S1 Data.