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Fig. 7 - Supplemental 1 Generation of locus-deletion mutants using CRISPR–Cas9. (A, C, E, G) Schematic representations show the genomic structure of zebrafish hoxb4a, hox5a, and hoxb5b. Boxes represent the exons, with the blue boxes indicating the coding regions. Black arrowheads indicate the target sites of crRNA, while red arrowheads represent the primers used for genotyping. (B, D, F, H) Display genomic locus deletions of hox4a, hox5a, hoxb5b, and hoxb4a-b5a deletion mutants. The flanking sequences of the 5′- and 3′-crRNA targets (underline) are shown in blue and green letters, respectively.
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