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Fig. 6 - Supplemental 1 Generation of the frameshift-induced hox mutants using CRISPR–Cas9. Schematic representations show zebrafish hoxb8b, hoxb5b;hoxb6b, and hoxb5b;hoxb6b;hoxb8b mutants. The other frameshift-induced hox mutants were previously described (Maeno et al., 2024). The nucleotide sequence around the mutations is shown on the left. The target sequence of crRNA is emphasized with an orange underline. The inserted nucleotide sequence is emphasized in red. Below the DNA sequence, the predicted amino acid is shown. Red letters indicate the abnormal amino acid caused by the frameshift mutation. On the right, a schema represents the predicted protein structure. The blue box indicates the homeodomain, and the red box indicates the abnormal amino acid sequences from the frameshift mutations. The total number of predicted amino acids is shown on the right of the schema.