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Fig. 5 Expression patterns of hox5a and hoxb5b are regulated by RA. (A) Schematic representation of the 49 hox genes organized into seven hox clusters in zebrafish. hox genes in hoxba and hoxbb clusters are highlighted with orange. (B) Expression profiles of the 49 hox genes in wild-type and raldh2−/− embryos at the 20-somite stage, analyzed by RNA-seq. The average FPKM of each hox gene was compared between sibling and raldh2 mutants. The absence of specific hox genes is indicated by a slash. (C–H) Expression patterns of hoxb5a and hoxb5b in sibling wild-type, raldh2−/−, and RA-treated raldh2−/− embryos at the 10-somite stage. Arrowheads indicate the presumptive positions of pectoral fin buds. For each genotype, reproducibility was confirmed with at least three different specimens. All images are captured from the dorsal side. Scale bars represent 100 µm.