Fig 6

Fig 6 KIF15 interacts with PSD95 and affect its localization in neuronal cytoplasm neurons of the PFC.

A and C: Co-IP result of cortical protein homogenate from P1 Kif15^+/+ mouse. B: Result of co-IP assays from 293T cells which simultaneous overexpression of KIF15-GFP and PSD95-Flag. Asterisks indicate interaction bands in the Western blot assays, triangles represent negative bands with no interaction. D: Immunofluorescent images showing the distribution of PSD95 expression in the PFC neurons at 3, 6 and 11 week-old mouse (Right panel, higher magnification of dashed boxed area). E: Wildtype and Kif15 knockout DIV18 neurons were immunostained for PSD95 and presynaptic synaptophysin. The colocalization of PSD95 and synaptophysin in confocal images was considered as a synapse (solid white arrows). F: The synaptic density in KIF15 knockout neurons was significantly lower compared to the wildtype group, n = 15 neurons per condition. All the data are presented as mean±SD, ***P < 0.001,two-tailed student’s t test between two groups.