Fig 2

Fig 2 Kif15 deficiency affects the expression of excitatory postsynaptic receptors in PFC and hippocampus.

(A and D): Western Blot analysis showed decreased protein levels of PSD95 and NMDA receptor GluN2A in the PFC of 3 week-old Kif15^-/- mice, n = 7 ~ 8 in Kif15^+/+ group, n = 6 in Kif15^-/- group. (B and E): Decreased expression of PSD95 in the PFC at 6 week-old Kif15^-/- group mice, n = 6 in Kif15^+/+ group, n = 8 in Kif15^-/- group. (C and F): Increased expression of PSD95 in the PFC of Kif15^-/- group compared with Kif15^+/+ group mice at 11 week-old, n = 7 in each group. (G and J): The expression of PSD95 in hippocampus of Kif15^-/- mice decreased at 3 weeks, n = 8 in Kif15^+/+group, n = 6 in Kif15^-/- group (H and K): The expression of PSD95 and GluN2A in hippocampus of Kif15^-/- mice was lower than that of wild type mice at 6 weeks, n = 6 in Kif15^+/+ group, n = 8 in Kif15^-/- group. (I and L): The expression of PSD95, GluA1 and GluN2A in the hippocampus of Kif15^-/- mice was significantly higher than that of Kif15 WT mice at 11 weeks, n = 7 in each group. All the data are presented as mean±SD, *P < 0.05, **P < 0.01, two-tailed student’s t test were used for comparison between two groups, expect for the GluA1 of J, which was used as a nonparametric test.