Fig. 3

Fig. 3 Oligodendrocyte-restricted expression of grm5a promotes myelin sheath elongation.

a, Construct used to express mGluR5 tethered to eGFP, alongside a membrane anchored reporter mScarlet, in myelinating oligodendrocytes. b, Control construct used to express fluorescent reporter in myelinating oligodendrocytes. c,c′, Images of oligodendrocytes expressing control mbp:memScarlet in wild-type (WT) (c) (N = 15) and grm5a^−/− (c′) (N = 20) animals at 4 dpf. d,d′, Oligodendrocytes expressing mbp:memScarlet-P2A–grm5a–eGFP in WT (d) (N = 12) and grm5a^−/− (d′) (N = 12) animals at 4 dpf. Scale bar, 10 μm. e, Relative frequency distribution of individual myelin sheath lengths. WT mbp:memScarlet n = 265 (mean 26.49 µm); WT mbp:memScarlet-P2A–grm5a–eGFP n = 124 (mean 31.55 µm); grm5a^−/− mbp:memScarlet n = 365 (mean 19.54 µm); grm5a^−/− mbp:memScarlet-P2A–grm5a–eGFP n = 138 (mean 31.58 µm). f, Mean sheath length of WT oligodendrocytes expressing mbp:memScarlet-P2A–grm5a–eGFP (N = 12, 32.19 ± 8.41) or mbp:memScarlet (N = 15, 26.92 ± 4.28) (two-sided unpaired t-test, P = 0.0445). g, Mean myelin sheath length of grm5a^−/− oligodendrocytes expressing mbp:memScarlet-P2A–grm5a–eGFP (N = 12, 33.25 ± 6.57) or mbp:memScarlet (N = 20, 20.05 ± 3.45) (two-sided unpaired t-test, P = <0.0001). h, Sheath number per oligodendrocytes expressing mbp:memScarlet-P2A–grm5a–eGFP (12.67 ± 3.46) or mbp:memScarlet (17.13 ± 5.17) (two-sided unpaired t-test, P = 0.0164). i, Sheath number per oligodendrocytes in grm5a^−/− mutants expressing mbp:memScarlet-P2A–grm5a–eGFP (N = 12, 11.25 ± 4.33) or mbp:memScarlet (N = 20, 17.1 ± 5.21) (two-sided unpaired t-test, P = 0.0027). Scale bar, 10 μm. Data show mean ± s.d.

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