FIGURE 8

FIGURE 8

Depletion of nucleophosmin 1a (npm1a) inhibits senescent cell accumulation and proliferation after NMDA damage. (A) Localization of npm1a expression by scRNAseq analysis after NMDA damage. (B) Depletion of npm1a by intravitreal injection of antisense oligonucleotides (ASOs). Control retinas were injected with either PBS or a mismatch control ASOs, and knockdown of npm1a was confirmed using qRT/PCR. (C) Depletion of npm1a inhibited accumulation and detection of senescent cells (SA-βgal⁺) at 3 dpi after NMDA damage. (D) Depletion of npm1a reduced detection of 4c4⁺ immune cells at 3 dpi after NMDA damage. (E, F) Depletion of npm1a in Tg[tuba1a.eGFP] zebrafish inhibited proliferation after NMDA damage at 3 dpi as measured by colocalization and counting of PCNA⁺/GFP⁺ MG-associated clusters in the Inner Nuclear Layer (INL) (n = 3–6, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001).