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Fig. 5 - Supplemental 1 Lysosome-rich enterocytes (LREs) show regional expression patterns and responses to the gut microbiome. (A) Dot plot shows KEGG pathways that were significantly upregulated in anterior LREs from the conventional (CV) dataset. Dot color corresponds to the adjusted p value, while dot size describes the number of pathway genes expressed in anterior LREs. (B) Dot plot of KEGG pathways that were significantly upregulated in anterior LREs of germ-free (GF) larvae. (C) Dot plot of KEGG pathways that were significantly upregulated in the posterior LREs of CV larvae. (D) Dot plot of KEGG pathways that were significantly upregulated in the posterior LREs of GF larvae. (E) Heatmap showing expression of KEGG pathway lysosome genes by LREs, midgut cell types and Pharynx, esophagus, cloaca 1 cells in the GF larvae. (F) Bar plots show the percentage of mCherry-positive and mCherry-negative cells that express components of the endocytic machinery in anterior LREs (top) and posterior LREs (bottom). (G, H) Volcano plot shows differentially expressed genes between anterior LREs and all other cell clusters in the CV (G) and GF (H) datasets. Genes that play a role in tryptophan metabolism are labeled. Red points with log2FC >0.5 are upregulated genes in anterior LREs from the CV (F) and GF (G) datasets. (I) Volcano plot shows differentially expressed genes between anterior and posterior LREs in the CV dataset. Tagged genes are peptidases. Red points with log2FC >0.5 and genes that were upregulated in anterior LREs, while red points with log2FC <–0.5 were upregulated genes in posterior LREs. (J, K) Volcano plots show differentially expressed genes between GF and CV anterior (J) and posterior LREs (K). Genes marked with red points with log2FC >0.5 were upregulated in the GF condition, while log2FC <0.5 were upregulated in the CV condition.