Fig. 4 qRT-PCR validation of gene expression changes in Klebsiella pneumoniae-induced zebrafish lung injury. (A) Validation of expression changes in lung development-related genes. (B) Validation of expression levels of genes involved in bacterial response. (C) Validation of expression changes in inflammation-related genes after infection. (D) Validation of expression levels of genes associated with the TGF-β signaling pathway. Relative expression levels were calculated using the ΔΔCt method, with data presented as mean ± standard deviation (n = 3). Statistical significance was determined using Student’s t-test (p < 0.05).
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