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Fig. 3 AGGF1 knockdown alters alternative splicing and identification of differential alternative splicing (DAS) events in 436 genes. (A) Diagram of typical alternative splicing (AS) events. A3, alternative 3′ splice sites; A5, alternative 5′ splice sites; AF, alternative first exon; AL, alternative last exon; MX, mutually exclusive exons; RI, intron retention; SE, skipping exon. (B) AS events identified by full-length cDNA sequencing in each sample of HPAEC transfected with AGGF1-specific siRNA (siAGGF1) and negative control siRNA (siNC). (C) Volcano plot of differential AS events in the siAGGF1 group compared with the siNC group. (D) Heatmap of DAS events. (E) Number of different types of DAS events vs. the number of associated genes. (F) GO enrichment analysis of genes involved in DAS events. Down, downregulated DASs; Up, upregulated DASs.