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Fig. 1 Nanopore full-length cDNA sequencing analysis identifies 1144 new genes in HPAEC. (A) Western blotting analysis of AGGF1 in HPAEC transfected with AGGF1-specific siRNA (siAGGF1) and negative control siRNA (siNC). β-Actin was used as a loading control. (B) Length distribution of non-redundant transcript sequences. The red dashed line indicates the length of N50. (C) Number of genes and transcripts identified in HPAEC. (D) UpSet plot of novel genes that can be annotated by eight functional databases. (E) Eukaryotic orthologous groups of proteins (KOG) annotation of novel genes identified in HPAEC. (F) Real-time RT-PCR analysis to validate the identification and differential expression of some unannotated genes in HPAEC transfected with siAGGF1 and siNC. (n = 3 samples/group; ***p < .001; ****p < 0.0001 vs. Control).