ZFIN Image: Zhou et al., 2025, Figure 5

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Figure Caption

Figure 5

Rescue experiments conducted on the asb5-deficiency group marked by the myl7 probe in embryos at 48 hpf. (A–C) Injection of asb5a-mRNA alone did not rescue the abnormal looping phenotype. (D–F) Injection of asb5b-mRNA alone also failed to rescue the abnormal phenotype. Images (G–I) show that the simultaneous injection of asb5a and asb5b-mRNA effectively rescued the phenotype in the asb5-deficiency group. Images (A′–I′) present magnified images of the heart, marked by the myl7 probe, from various groups of embryos at 48 hpf. red E: eyes; white V: ventricle; red A: atrium; D-loop: dextral looping; N-loop: no looping; L-loop: levo-looping; Scale bar = 100 µm (belly up view).

Acknowledgments

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