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Fig. 3 - Supplemental 5 Phenotype of aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 double mutant at 28 hpf and 3 dpf. The same aqp1a.1+/rk28;aqp8a.1+/rk29 heterozygote and aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 homozygote embryos were imaged at 28 hpf and 3 dpf. (A–D) Brightfield images of 28 hpf (A, C) and 3 dpf (B, D) embryos showing normal gross morphology of aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 embryo. (E–H) Maximum intensity projections of confocal z-stacks of the brain vasculature of 28 hpf (E, F) and 3 dpf (G, H) embryos. (I–M) Maximum intensity projections of confocal z-stacks of the trunk vasculature of 28 hpf (I, K) and 3 dpf (J, L, M) embryos. The trunk and tail were imaged separately and stitched using Fiji stitching plugin to make an entire image. A magnified image of the boxed areas in (K) and (L) is shown in (M). In panel (K), numbers in red correspond to those sprouts (ISV5, ISV6, ISV9, ISV11, ISV13, and ISV15) that will not form complete ISVs at 3 dpf (asterisks in M). Scale bar, 200 µm.