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Fig. 3 - Supplemental 4 ISV phenotype classification in aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 mutant embryos at 3 dpf. (A) Class I (76.3% of truncated ISVs). vISV crosses horizontal myoseptum and turns right (posteriorly toward the tail) along the ventral side of the spinal cord. Vessel comprises four ECs. (B) Class II (14.3% of truncated ISVs). Absence of ISV in segmental position (sometimes a single EC is present, which is neither connected to the DA nor DLAV, asterisk). (C) Class III (1.3% of truncated ISVs). vISV does not cross horizontal myoseptum and aISV is not connected to the DA. (D) Class IV (2.8% of truncated ISVs). aISV is not connected to the DA. (E) Class V (2.3% of truncated ISVs). aISV crosses horizontal myoseptum, but has no connection to DLAV. Vessel comprises three ECs. (F) Class VI (3.1% of incomplete ISVs). vISV extends over the notochord and spinal cord, but does not connect to DLAV. In panels (A, C, E), dashed line marks an approximate position of horizontal myoseptum. DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; EC, endothelial cell; ISV, intersegmental vessel; aISV, arterial ISV; vISV, vein ISV; NC, notochord; PCV, posterior cardinal vein; SC, spinal cord. Scale bar, 20 µm.