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Fig. 3 - Supplemental 3 Analysis of ISV defects in aquaporin mutant embryos. (A–D) Number of truncated ISVs per embryo in wildtype (A, n = 47), aqp1a.1rk28/rk28 (B, n = 30), aqp8a.1rk29/rk29 (C, n = 38), and aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 (D, n = 204) larvae at 3 dpf. (E) Anterior-to-posterior distribution of truncated ISVs in the trunk and tail region. (F–H) Maximum intensity projection confocal z-stacks of aquaporin heterozygote and homozygote embryos at 28 hpf. Images are representative of 10 embryos for each genotype. Scale bar, 200 µm. (I–K) Quantification of ISV length at each position along the length of the embryos in aquaporin heterozygote and homozygote embryos at 28 hpf; data is presented as mean ± SD. ISVs, intersegmental vessels.