|
Fig. 3 Loss of Aquaporin function leads to defective trunk vessel formation. (A–D) Representative maximum intensity projection confocal z-stacks of 28 hpf aqp1a.1+/rk28;aqp8a.1+/rk29 (A), aqp1a.1rk28/rk28 (B), aqp8a.1rk29/rk29 (C), and aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 (D) embryos. (E) Quantification of ISV length at different positions along the trunk in 28 hpf embryos (aqp1a.1+/rk28;aqp8a.1+/rk29: n = 21 embryos; aqp1a.1rk28/rk28: n = 20 embryos; aqp8a.1rk29/rk29: n = 20 embryos; aqp1a.1rk28/rk28;aqp8a.1rk29/rk29: n = 19 embryos from two independent experiments); data is presented as mean ± SD. (F) Illustration of aISV and vISV connections in the trunk of wildtype embryo at 3 dpf. (G–J) Representative maximum intensity projection confocal z-stacks of 3 dpf wild type (G), aqp8a.1rk29/rk29 (H), aqp1a.1rk28/rk28 (I), and aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 (J) embryos. Asterisk marks incomplete ISVs, arrows point to EC nuclei on the contralateral side. (K) Pie chart showing the proportion of different classes of truncated ISV phenotypes (I–VI) found in aqp1a.1rk28/rk28;aqp8a.1rk29/rk29 mutant embryos at 3 dpf. DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; aISV, arterial ISV; vISV, venous ISV; NC, notochord; NT, neural tube; PCV, posterior cardinal vein. Scale bar, 50 µm (G–J) and 200 µm (A–D).