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Figure Caption
Fig. 1 The antigen of zpr-3 is zebrafish Rho protein. (A) The ladder-like bands produced by zpr-3 and the anti-Rho antibody in the western blot assays of wild-type zebrafish protein lysates. (B) Full-length zebrafish Rho protein tagged with Flag was expressed in ARPE-19 cells and detected by western blot using the zpr-3, anti-Rho and anti-Flag antibodies, respectively. The images of western blot are shown. (C) Overlapping of the fluorescence signals of zpr-3 and anti-Flag antibodies in ARPE-19 cells transfected with the zebrafish rho expression plasmid. Scale bar, 20 μm.
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