ZFIN Image: Vassalli et al., 2024, Figure 4

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Figure 4

Cell proliferation and apoptosis assays on zebrafish oc morphant retinal cells. (A–E) Immunostaining for the mitotic marker PH3 in (A) Eye of control embryo injected with STD MO and (B–E) of embryos injected with oc1, oc2, oc-like and triple oc-MO. (F) Graphical analysis of proliferating cells in oc1, oc2, oc-like and triple oc-MO embryos at 24 hpf with respect to STD MO embryos. (G–L) Tunel assay showing no significant differences in the number of apoptotic cells (black circles) between control (G) and oc1, oc2, oc-like and triple oc-MO eyes (H–K). (L) Graphical analysis of the number of apoptotic cells observed in (G–K) retinas. Data are expressed as mean ± SEM. Non parametric Kruskal–Wallis test with Dunn’s post hoc correction. *** p < 0.001.

Acknowledgments

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