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Fig. 8 Startle response of 5 dpf zebrafish larva. A: comparative response of wild-type (AB) larvae, myo7aa marty220d/ty220d homozygous (myo7aa−/−) larvae and marty220d/+/mar+/+ siblings (myo7aa+/?). B: comparative response of wild-type (AB) larvae, KO tubB4b[Chr5]+/+, KO tubB4b[Chr5]+/− and KO tubB4b[Chr5]−/− larvae. C–D: comparative response of wild-type (AB) larvae, Tg(tubB4b[Chr5]:TubB4b[Chr5]-EGFP)+/0; KO tubB4b[Chr5]+/− and Tg(tubB4b[Chr5]:TubB4b(R391H)[Chr5]-EGFP)+/0; KO tubB4b[Chr5]+/− larvae. Results from two different transgenic lines are presented for wild-type (#8 and #25) and R391H mutation (#4 and #5). Sound stimulation was performed for 100ms at 450Hz frequency with increasing intensities. Graphs represent the startle response score of larvae from various genotypes. Score values range from 0 in absence of response to 1 when one larva responded to all 12 consecutive sound stimuli at one given intensity. Number of larvae is indicated next to genotype. Statistical comparison was made between larvae of all different genotypes at each sound intensity. Only significant differences are marked as follows: ∗P < 0.01 vs wild-type AB; ∗∗P < 0.001 vs wild-type AB; §P < 0.001 vs myo7aa +/?
Reprinted from Developmental Biology, 517, Smaili, W., Pezet, C., Marlin, S., Ernest, S., R391 human dominant mutation does not affect TubB4b localization and sensory hair cells structure in zebrafish inner ear and lateral line, 301-316, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.