Figure 10

Figure 10

Expression levels of cytokines and macrophage markers in zebrafish larvae infected with Mycobacterium marinum determined by RT-qPCR. The Tg(mpeg1:mCherry)gl23 zebrafish larvae, infected (+) or non-infected (−) with M. marinum, were treated with podocarpusflavone A at 32 µM (PCFA32), rifampicin at 32 or 100 µM (RIF32 and RIF100) or were left untreated (CTRL) for 5 days, and relative expression of mRNA was measured for IL-12, IL-6, TNF-α, IL-10, and CXCR3.2. The expression of each target gene was related to the expression of the housekeeping ppial gene. The data were expressed as the mean ± SEM. * p < 0.05 and ** p < 0.01 compared to the CTRL infected group and # p < 0.05, ## p < 0.01 and ### p < 0.001 as compared to the CTRL non-infected group.