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Fig. 5 Morphometric (A,B) and behavioral assessment (C,D) of wild−type (WT), Tq206+/−, and Tq206−/− zebrafish embryos after 48 h treatment with 0.05 μM CFTR corrector C17. (A) Total body length differences of treated and untreated WT, Tq206+/− and Tq206−/− zebrafish embryos. (B) Tail curvature angle measurements comparison with treated and untreated WT, Tq206+/− and Tq206−/− zebrafish embryos. Qualitative (C) and quantitative (D) analyses of the distance moved by treated and untreated WT, Tq206+/− and Tq206−/− zebrafish embryos. Embryos underwent 30 min of acclimatization followed by two alternating cycles of 10 min of darkness and 10 min of illumination, with the dark phases being indicated by grey boxes. All zebrafish embryos were 3 dpf old, and the treatment group received treatment of CFTR corrector C17 at 0.05 μM concentration for 48 h. Quantitative data are presented as mean ± SEM. Statistical analysis was performed by the Mann-Whitney test; **** p < 0.0001.