Figure 2

Figure 2

Time-dependent evolution of electroencephalographic changes recorded from the brain of zebrafish larvae exposed to pilocarpine (PILO). Larvae was incubated in medium containing PILO (50 mM) for 5 min, and, afterwards, LFPs were recorded for 20 min: (A) 4 min after start of recording, no clear-cut seizure-like events are present; (B) 8 min after start of recording, short ictal and interictal events are seen; (C) 12 min after start of recording; progression of seizure-like ictal and interictal activity is evidenced (D) 14 min after start of recording, further progression and intensification of seizure-like ictal and interictal activity is shown; (E) 18–20 min after start of recording, prolonged and continuous convulsion-like activity.