Fig. 6

Fig. 6

Snail but not EGFR inhibition prevents YB‐1‐induced PM cell migration. (A) Cells were exposed to 10 μm erlotinib (Erlo) or DMSO (Co) and after 24 h treated with 100 ng·mL⁻¹ doxycycline (+dox) as indicated. Cells were transfected with 10 nm (15 nm for Ren^YB1‐i) of (B) EGFR‐specific (si‐EGFR), (C) SNAI1‐specific (si‐SNAI1) or control (si‐Co) siRNA and on the next day treated with 100 ng·mL⁻¹ doxycycline. Cumulative migrated distance was measured via live cell videomicroscopy for 48 h. Experiments were performed two times in quadruplicates. Quantification was performed using manual single cell tracking of > 20 cells per group in imagej. Each dot represents one cell, and the horizontal lines indicate the mean. ANOVA and Tukey's multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant.