Fig. 4

Fig. 4

YB‐1 levels directly affect PM cell migration. Cells were transfected with 5 nM of si‐YB1^CDS (si‐CDS), si‐YB1^UTR (si‐UTR) or control siRNA (Co) and on the next day treated with 100 ng·mL⁻¹ doxycycline (+dox). (A) Log2 fold change of YBX1 mRNA levels after 48 h. Each dot represents the mean of one cell line, derived from 3 biological replicates performed in duplicates. Data are shown as mean ± SEM. (B) Representative pictures and YB‐1 protein levels relative to control‐siRNA‐transfected cells (Co) derived from densitometric analysis of western blots (N = 3). Data are shown as mean ± SEM. (C) Cumulative migrated distance of single cells after siRNA transfection and treatment with doxycycline (+dox) as indicated within 72 h. Quantification was performed using manual single cell tracking in imagej. Each dot represents one cell and the horizontal lines indicate the mean. ANOVA and Tukey's multiple comparisons test. **P < 0.01, ***P < 0.001.