|
Fig. 2 The chemical structure of EN and effects of EN on skull mineralization in zebrafish larvae. (a) Chemical structure of EN. (b) Zebrafish larvae were treated with EN (0.2, 1, and 5 μM), and skull mineralization ability was determined using ARS. (c) Mineralization areaJOTr-D-22-00514R1. (d) Mineralization IOD. (e) The mRNA expression of osteoblast-related genes (ALP, RUNX2a, SP7, and OCN) on 9-dpf in zebrafish larvae. The data are shown as the means ± SD (n = 10 zebrafish larvae/group). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. Con. Scale bar: 100 μm. EN, eurycomanone; ARS, alizarin red S; IOD, integrated optical density; ALP, alkaline phosphatase, RUNX2a, runt-related transcription factor 2a; SP7, Sp7 transcription factor; OCN, osteocalcin; dpf, days after fertilization; Con, Control. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)