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Yu et al., 2023 - Loss of dlx5a/dlx6a Locus Alters Non-Canonical Wnt Signaling and Meckel's Cartilage Morphology

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Figure 6

Expression of non-canonical Wnt signaling components are altered in dlx5i6^−/− larvae during MC morphogenesis. (A–D) Expression of wls at 2 dpf (A,B) and 3 dpf (C,D) by WISH. (E–H) Expression of wnt5b at 2 dpf (E,F) and 3 dpf (G,H) by WISH. Scale = 100 μm. (I–K) Normalized relative expression of wnt5b (I), frzd7a (J) and ror2 (K) by RT-qPCR using at least 4 pools of head cDNA. All data presented as mean ± SEM. * p < 0.05, ns = no significance (wnt5b at 3 dpf: p = 0.21; frzd7a at 2 dpf and 3 dpf: p = 0.07, p = 0.28, respectively; ror2 at 2 dpf and 3 dpf: p = 0.13, p = 0.08, respectively).

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