Fig. 6

Fig. 6 RBI- and CHT-derived neutrophils have different responses to resection.

A Heatmap of the top 5 markers in primitive and definitive neutrophils at steady state (complete list available in Supplementary Data 2, filtered on a minimum detection fraction threshold = 0.1, log fold-change threshold = 0.25 and p-value < 0.05 (not adjusted), negative binomial test, out of the 2000 most variable genes, only genes with positive fold change were selected). B, C Heatmaps of the top 15 upregulated and downregulated genes upon tail resection among the RBI- derived (B) and CHT-derived (C) neutrophil populations (complete list available in Supplementary Data 3 and 4, filtered on a minimum detection fraction threshold = 0.1, log fold-change threshold = 0.25 and p-value < 0.05 (not adjusted), negative binomial test, out of the 2000 most variable genes, only genes with positive fold change were selected). D Venn diagram showing the number of overlapping upregulated and downregulated genes between RBI-derived and CHT-derived neutrophils. E Network of the pathways associated with upregulated and downregulated genes among RBI-derived and CHT-derived neutrophils upon tail resection. Each gene associated with a given pathway is displayed. Pie charts indicated the relative contribution of each type of neutrophil to that term/gene (see Methods). Note that csf3b appears both as associated with chemotaxis upon resection and as a specific marker of the RBI-derived neutrophils when compared with CHT-derived neutrophils. Heatmaps show genes with p-value < 0.05, based on a negative binomial distribution, out of the 2000 most variable genes.