Figure 8

Figure 8

Wnt signaling in the skin of zebrafish. (A) Immunohistochemical staining against the Wnt-reporter mCherry and nuclear counterstain with DAPI. From left to right: fish underwent 21 days of DMSO, 21 days of prednisolone, 17 days of DMSO followed by 4 days of prednisolone and 17 days of prednisolone followed by 4 days of DMSO treatment. Scalebar 50 µm. (B) Quantification of the 7xTCF-Xia.Siam:nlsmCherry Wnt reporter expression in the skin. Lines represent mean ± SEM of mCherry signal intensity for fish that underwent 21 days of DMSO, 21 days prednisolone, 17 days DMSO followed by 4 days prednisolone and 17 days of prednisolone followed by 4 days of DMSO treatment. Signal was measured from the outermost layer towards the inner layers of the skin. Average skin thickness is around 50 µm. n=5 (4 females, 1 male in DMSO 21d, DMSO 17d Pred 4d, Pred 17 d DMSO 4d; 5 females in Pred 21d) in all groups with 2 sections per individual.