FIGURE 2

FIGURE 2

SAL has bacteriostatic activity in vivo but not in vitro. (A) Images showing the growth of M. marinum on 7H10 plates containing different concentrations of INH and SAL after 8 days incubation. (B) Statistics of the colony number in (A). (C) Representative images for the distribution of M. marinum (Wasabi) in different groups of zebrafish at 72 hpi. Scale bar: 200 μm. (D) Statistics of integrated green fluorescence intensity in (C). Values are shown as mean ± s.e.m. One-way ANOVA for comparison at the same time point. Two-way ANOVA for co-operation at different time points. (E)M. marinum-Wasabi-infected RAW cells with or without SAL treatment. (F) Statistics of relative fluorescence units (RFU) of total (extracellular and intracellular) bacteria in (E). A and B are one representative data from three independent repeats. C and D are one representative data from two independent repeats. E and F are one representative data from three independent repeats.