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Fig. 5; supplement 1

Jag1a intracellular domain does not have an effect on notochord cell fate.

(A–B) Optical sections of 2 dpf zebrafish that were injected with the emilin3a:GFP or emilin3a:GFP-p2a-JICD constructs. (C) Proportion of vacuolated cells at 2 dpf. This proportion was calculated by counting the number of vacuolated cells divided by the sum of the number of sheath and vacuolated cells. Each point represents an independent fish quantified from on z-stack confocal acquisitions (n = 9 GFP, n = 6 GFP-jag1aICD). (n.s.) p-value > 0.05 by two-tailed t-test. Scale bars, 50μm.

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