IMAGE

Fig 5

ID

ZDB-IMAGE-210820-24

Genes

irf2bp2a

Publication

Gao et al., 2021 - Irf2bp2a regulates terminal granulopoiesis through proteasomal degradation of Gfi1aa in zebrafish

All Figures

Figures for Gao et al., 2021

Image

Figure Caption

Fig 5 <italic toggle='yes'>Irf2bp2a</italic> gene is repressed by Gfi1aa.

(A) Luciferase reporter assay. Bars showed the relative luciferase activity on the zebrafish irf2bp2a promoter (-2.1 kb) (Student t test, N = 3. Error bars represent mean ± SEM. ***P < 0.001). (B) CHIP-PCR analysis of irf2bp2a promoter in zebrafish embryos expressing GFP, Gfi1aa-GFP or Gfi1aa-Δzinc finger-GFP using an anti-GFP antibody. The statistical significance was calculated by using one-way ANOVA. The asterisk indicates a statistical difference (N = 4. Error bars represent mean ± SEM. ns: not statistically significant, ****P < 0.0001). (C) irf2bp2a relative expression level analyzed by quantitative PCR in GFP-positive cells sorted from control Tg(mpx:eGFP) embryos and gfi1aa MO injected Tg(mpx:eGFP) morphants at 2 dpf (Student t test, N = 5. Error bars represent mean ± SEM. ****P < 0.0001).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.