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Figure 4

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ZDB-IMAGE-210327-29

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Brown et al., 2021 - PINK1 deficiency impairs adult neurogenesis of dopaminergic neurons

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Figures for Brown et al., 2021

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Figure 4

DA neurons in the adult PVO are generated from Her4-expressing progenitors. (A–B) Fluorescent MIP of the PT of double transgenic (Tg(her4:ERT2CreERT2); Tg(ubi:loxGFPloxmCherry)) 3 month zebrafish injected with tamoxifen. Cell nuclei are labelled with DAPI (blue), DA neurons with Th1 (green) and Her4 progenitors with mCherry (red) throughout. Th1 is shown as a single channel in (ii), mCherry is shown in (iii). Th1 (green) and mCherry (red) are shown together in (iv). Boxed regions show magnified image of the double labelled cell. (Ai–iv) TPp: mCherry and Th1 mark adjacent cells but are not co-localised (yellow arrows). (Bi–iv) PVO: Double staining shows co-localisation of mCherry and Th1. The presence of mCherry + Th1 + cells in the PVO suggests that newly generated DA neurons are derived from Her4 progenitors in this neuronal subpopulation. Yellow dashed line indicates ventricle.

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