FIGURE 3

FIGURE 3

Neuroserpin deficient zebrafish showed reduced locomotor activities and more neurons loss in diencephalon area under CoCl₂ induced hypoxic injury. (A) Representative graph for distance traveled in 40 min for WT and serpini1^−/− zebrafish. (B) Quantification of average distance moved per minute in WT and serpini1^−/− under 10 mM CoCl₂ induced injury. The data are presented as the mean ± SEM, n = 96 per group. Statistical analyses were performed with Student’s t-test, ***p < 0.001, ns: no significant, compared with WT in the same CoCl₂ concentration. (C) Images of neurons in diencephalon in Tg (Huc: RFP) and Tg (serpini1^−/−-HuC: RFP) under 10 mM CoCl₂ induced injury. Scale bar:100 um. (D) Quantification of average fluorescence intensity of neurons in diencephalon area in Tg (Huc: RFP) and Tg (serpini1^−/−-HuC: RFP) under 10 mM CoCl₂ induced injury. The data are presented as the mean ± SEM, n = 6 per group. Statistical analyses were performed with Student’s t-test, **p < 0.01, ns: no significant, compared with WT in the same CoCl₂ concentration.