Figure 6.

Figure 6.

Measurement of metastatic spread of MCF7 and MDA-MB231 cells in zebrafish larvae in vivo models Human RFP-MCF7 (left) or RFP-MDA-MB-231 (right) cells cultured with conditioned media from different conditions, were injected into the perivitelline space of 2-day-old zebrafish larvae [Control (vehicle MCF-7 cells, alone), TCDD (MCF-7 cells treated with 25 nM TCDD), co-culture (MCF-7 co-cultured with hMADS), and coexposure (co-culture with TCDD)]. Fish were imaged 24 h later at 8.5× magnification by fluorescence microscopy. (A) Representative images from 21–46 fish/group. (B) Quantitation of (a) the number of fish with one or more metastases and (b) the average number of metastases/fish+SE. Graph represents means±SEM of three measurements. The numerical information mean±SEM and p-values are provided in Table S8. (Kruskal–Wallis’s H test (nonparametric comparison of k independent series) followed by a 1-factor ANOVA test (parametric comparison of k independent series, * p<0.05). (C) The percentage of fish with metastasis in the head region. Each experiment was repeated in triplicate (MCF-7) or quadruplicate (MDA-MB-231) with 7–12 fish per condition in each experiment. Note: ANOVA, analysis of variance; SEM, standard error of the mean; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.