IMAGE

Figure 4

ID

ZDB-IMAGE-210113-13

Genes

olig2

Publication

Danesin et al., 2021 - Sulf2a controls Shh-dependent neural fate specification in the developing spinal cord

All Figures

Figures for Danesin et al., 2021

Image

Figure Caption

Figure 4

The Nkx2.2a/p3 domain is dorsally expanded at the expense of the Olig2/pMN domain in 24 hpf sulf2a-depleted embryos. Side views of 24 hpf embryos. (a–c”) Double detection of olig2 mRNA and Sox2 in Tg(nkx2.2a:mEGFP) embryos injected with ctrlMO (a–a”), sulf2aMO^ATG (b–b”) or sulf2aMO^splice (c–c”). Vertical sets present successively the nkx2.2a:mEGFP signal (green in a”–c”), the olig2 mRNA staining (red in a”–c”) and the merged image together with Sox2 staining (blue in a”–c”). Note the dorsal expansion of the nkx2.2a:mEGFP signal associated with a strong decrease in the olig2 signal in sulf2a morphant embryos. (d, e) Quantification of nkx2.2a:mEGFP + /Sox2 + (d) and olig2 + /Sox2 + (e) progenitors in embryos injected with ctrlMO (n = 27), sulf2aMO^ATG (n = 14) and sulf2aMO^splice (n = 23) from two independent experiments. Datasets were compared with Mann Whitney’s test (two-tailed). Data are presented as a mean number of cells along the dorso-ventral axis per embryo ± s.d (****p < 0.0001).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.