Figure 3—figure supplement 1.

Figure 3—figure supplement 1. Cohesin-deficient cells show altered nucleolar morphology.

(A) Representative images of nucleolar morphology as determined using fibrillarin (FBL) as a marker of nucleoli. (B,C) Quantification of FBL intensity and number of FBL spots per cell in each genotype. A minimum of 1000 cells was examined per individual experiment, n = 3 independent experiments, mean ± s.d., one-way ANOVA: *p≤0.05; **p≤0.01; ***p≤0.0005. (D) Types of nucleolar morphology observed in MCF10A parental cells. (E) Representative images of nucleolar morphology determined by using nucleolin (NCL) as a marker. (F) Quantification of NCL intensity per cell in each genotype. At least 500 cells were examined per individual experiment, n = 3 independent experiments, mean ±s.d., one-way ANOVA: **p≤0.01; ***p≤0.0005. (G) Quantification of NCL morphology classes present in cohesin-deficient cells. A.U., Arbitrary unit.