ZFIN Image: Esa et al., 2020, Figure 2

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Figure Caption

Figure 2

Basal MetAp2 activity and expression in LECs compared with VECs. (A) The MetAp2 mRNA levels in LECs (HMVEC-dLyAd cells) is ~60% of the mRNA levels in VECs (HUVECs), as measured by qRT-PCR (5 μg), n = 3. (B) Western blot analyses for determining the expression of MetAp2 in both cell lines. (C) Measurement of the basal activity of MetAp2 in HUVECs and HMVEC-dLyAd cells. A homogenate containing an equal number of cells was added to an L-Met-AMC solution (250 µM). The reaction was monitored for 1 h via the fluorescent quantification of the cleaved substrate. MetAp2 in HMVEC-dLyAd cells showed over a 65% reduction in the enzymatic ability to cleave labeled methionine when compared with HUVECs. The results were normalized to the total cell number. *** p < 0.001. The results are presented as mean ± SEM.

Acknowledgments

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