Specification and development of motoneurons are unaffected in maternal-zygotic ypel3 mutants.
(A-F) Analysis of the pMN domain and its derivatives. Transverse sections. (A-C) WT. (D-F) maternal zygotic (mz) ypel3-/-. (A, D) pMN domain and its derivatives labeled by olig2:kaede transgene. (B, E) Topro nuclear labeling. (C, F) Merge. (G-J) Analysis of motoneuron development in mz ypel3 mutants. Lateral views. Motoneurons are labeled by the mnx1:gfp transgene. (G-H) WT embryo at 26 hpf (G) and larva at 52 hpf (H). (I-J) mz ypel3 mutant at 26 hpf (I) and larva at 52 hpf (J). Images were taken at the level of somites 6 to10. (K-L) Quantification of the number of motoneurons at 26 hpf (K) and at 52 hpf (L). Bars show +/- SEM. Scale bars: 5 μm in A-F, 25 μm in G-J.
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