Fig 6

Fig 6

Mu4127 embryos expressing Gal4 in rhombomeres 3 and 5 were injected with H2B-citrine:UAS (A-C), H2B-citrine:UAS:atoh1a (D-F) or H2B-citrine:UAS:atoh1b (G-I) constructs in order to ectopically express the gene of interest in r3 and r5. Injected embryos were assayed for Citrine expression (green) and atoh1a (A-A’, D-D’, G-G’), atoh1b (B-B’, E-E’, H-H’) or lhx2b (C-C’, F-F’, I-I’) expression (magenta). Reconstructed transverse views displaying the merge of the red and green channels (A-I), or only the red channel (A’-I’). Note that ectopic expression of atoh1a in more ventral domains induces atoh1b and lhxb2 expression (see white arrowheads in D-F, D’-F’), whereas ectopic atoh1b expression induces lhx2b but not atoh1a (see white arrowheads in H-I, H’-I’). See Table 3 for numbers of analyzed embryos. r, rhombomere. Scale bars correspond to 50 μm.